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Cryoprotectant toxicity follows a similar pattern to chilling sensitivity with later stages being less sensitive to cryoprotectant Zhang et al. Embryos Cryopreservation of embryos has become an integral part of assisted reproduction. This prevents the problem, for example, of collecting ripe eggs, but not having sperm available to fertilize them. Cryopreservation of early stage zebra fish oocytes using the controlled slow freezing has been reported by Tsai et al. Finally, cryopreserved sperm of aquatic species will at some point become an entirely new industry itself. This can lead to a breeding matrix where a group of select males can be mated with a group of select females to develop populations with distinctive traits.

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